Still Got the Blues

Oh my..we didn't go "anywhere" this weekend, I mean outside from our town. Kimi's mummy not in the mood to go anywhere since a bit emotionally involve with the last part of the pee-hech-dee project and it is like taking a roller coaster ride. I feel physically and mentally drain. Thanks to my hubby..who help me a lot in taking care of me and kimi.

The project 'menguji kesabaran tahap maksima 9 skala richter'. It takes at least 5 days to visualise the data or output and several days for analysis or in my case several months. I had tried this since last year but there were many hiccups like late arrival of chemicals (kat sini pun ada masalah birokrasi), unknown shelf life of the chemicals and finally found through a hard way that most of the chemicals already expired plus juggling two big experiments at two different labs (at different locations and I only rely on public transport to commute to two places on certain days). Well, my project can be classified into three sections and I'm now in the last section. Luckily I managed to end the second section of the experiments early this year so I just have to concentrate on this last part but often my Thursdays are hijacked by a department and group meetings.

But last week I got a really good protein separations under my two dimensional gel profile. I couldn't show it here, most probably there is a new discovery in it? Who knows. It's like serendipity kind of thing. The truth it, I thought that the experiment won't work at all since I used to apply constant temperature using a water bath circulating system at a certain degree for the first dimension run (using a specific equipment). It took 48 hrs to complete, I ran them on Wed. at my co-supervisor's crony lab in the hospital, and left it to ran itself on Thursday, come Friday I wanted to switched the machine off and I was dumbfounded that water was totally leaking. The enviromental temperature must be fluctuate a bit but not exceeding the temperature that I already sat up. I just have to repeat another experiments that will include the same sample to be able to do the stats analysis. Gee..but I had to find out what's the temperature on that particular Thursday to run for this week experiment. Wish me luck! I need two weeks to run all these samples (must be optimistic) before I can concentrate doing the analysis with the latest software..ohw please..this is like more than 4th repeat per sample and I got 10 of them (looks not much but hmm will explain later about what's my project is all about in later post). Thank God my supervisor's didn't complain about my spending on chemicals..who's fault dumping me at his crony or subordinate lab (I'm the only one working in this field there, the rest are dealing with pathology servicing labs).

One of the equipment for separating the proteins in the biological sample (I used ovarian cancer cells). Can equip 6 of 2Dimensional gels. It's also one of the machine that can be used to muscle building since it's heavy when filled with solution. Look like an aquarium eh..berat pun macam aquarium.